Care and housing of mice in specific pathogen free barrier- $1.00/cage/day.
The mice are housed in micro-isolator cages on hepa-filtered racks; cages, food, and water are all autoclaved: weekly change-outs are performed within a biosafety cabinet.
Generation of transgenic mice or rats by pronuclear injection- $1815 plus $1.00/cage/day for cages resulting from embryo transfers.
By this process, approximately 30 potential founder mice or rats will be generated. Currently, our success rate is around 12 %. If, however, following genotyping, there are no transgenics, a second series of injections will be done at no cost to the investigator (except for the per diems).
Gene targeting in mouse embryonic stem cells- $1850 per construct.
Due to the unpredictable nature of different targeting constructs, there is no guarantee that any correctly targeted clones will be obtained.
Generation of chimeras by ES cell/blastocyst injection- $1815 plus $1.00/cage/day for cages resulting from embryo transfers.
Cryopreservation of mouse embryos- $65 for harvesting embryos and $65 for freezing; per session.
Long term storage of cryopreserved embryos- $20 per cane per year (note: one cane holds up to 8 straws).
Recovery of cryopreserved embryos- $85 for one transfer; and $45 for each additional transfer.
In addition, cages generated for housing of embryo recipients will be charged to the investigator at our standard per diem rate, $1.00/cage/day.