Arlen Johnson

Johnson, Arlen
Associate Professor In Molecular Genetics & Microbiology

E-mail: arlen@mail.utexas.edu

Website: http://www.bio.utexas.edu/faculty/ajohnson/index.htm

Main Office: NMS 1.126
Phone: 475-6350

Alternate Office: NMS 1.246
Phone: 475-9729

Mailing Address:
The University of Texas at Austin - ICMB
1 University Station A5000
100 E. 24th St.
Austin, TX 78712-1095

Graduate Students:

  • Bussiere, Cyril
  • Hung, Nai-Jung
  • Lo, Kai-Yin
  • Sardana, Richa
  • White, Joshua

    • Research Summary:
       My lab studies ribosome export and mRNA degradation in yeast. We use techniques spanning genetics, cell biology, molecular biology, biochemistry and proteomics. Recently, we identified the nuclear export pathway for the large ribosomal subunit. This pathway depends on Crm1p, the receptor for leucine-rich nuclear export signals, and the 60S-binding protein Nmd3p that acts as an adapter for export. Current work involves characterization of the export complex, characterization of the nascent 60S subunit at different stages of nuclear export and regulation of the export pathway. Our work on mRNA degradation has focused on the enzymes responsible for 5’ and 3’-degradation. We have recently shown that inhibition of the 3’-pathway by cis acting elements within an mRNA is sufficient for efficient translation of poly(A) minus mRNA in wild-type cells. Current work examines the biochemical function of the heterotrimeric Ski2/3/8 complex required for 3’-decay and the functional and physical interaction of this decay system with other decay pathways and with the translation apparatus.
     
    Publications:
    The specialized cytosolic J-protein, Jjj l, functions in 60S ribosomal subunit biogenesis (2007) PNAS U.S.A. 104, 1558-63.
    Mapping the functional domains of yeast NMD3, the nuclear export adapter for the 60 S ribosomal subunit (2007) J Biol Chem 281, 36579-36587.
    Novel interaction of the 60S ribosomal subunit export adapter Nmd3 at the nuclear pore complex (2007) J Biol Chem 282, 14028-14037.
    Mutational analysis of the ribosomal protein Rpl10 from yeast (2007) J Biol Chem 282, 32630-32639.
    Nuclear recycling of the pre-60S ribosomal subunit-associated factor Arx1 depends on Rei1 in Saccharomyces cerevisiae (2006) Mol Cell Biol 26, 3718-3727.
    Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p (2005) RNA 11, 1291-1302.
    Defining the order in which Nmd3p and Rpl10p load onto nascent 60S ribosomal subunits (2005) MCB 25, 3802-3813.
    Release of the export adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p and the cytoplasmic GTPase Lsg1p (2005) EMBO 24, 567-579.
    Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large ribosomal subunit (2001) J Cell Bio 151, 1057-1066.

     
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