George Georgiou

Georgiou, George
Professor in Chemical Engineering
Cockrell Family Regents Chair in Engineering #9

E-mail: gg@che.utexas.edu

Website: http://www.che.utexas.edu/georgiou/home.htm

Main Office: CPE 4.410
Phone: 471-6975

Alternate Office: MBB 3.312AA
Phone: 232-4104

Mailing Address:
The University of Texas at Austin - Chemical Engineering
1 University Station C0400
200 E. 26th St.
Austin, TX 78712-1095
Research Summary:
   Dr. Georgiou’s group is working on both biotechnology and mechanistic biology problems. In biotechnology, his group is focusing on the development of protein engineering and combinatorial library screening technologies for facilitating the discovery of new therapeutic antibodies and enzymes. Current projects include the development of therapeutic antibodies and "humanized" enzymes for medical applications. These studies are accompanied by detailed analysis of protein structure function to aid the understanding of antibody-antigen recognition and of enzymatic catalysis. In related studies, Dr. Georgiou’s group is developing technologies for the preparative expression of recombinant proteins in bacteria and other host cells. About half of Dr. Georgiou’s group is working on problems related to fundamental mechanistic issues in protein biogenesis. These include; (1) oxidative protein folding and redox control in bacteria; (2) the translocation of proteins across the membrane via the Twin Arginine Transporter (Tat) pathway; (3) Biogenesis and expression of membrane proteins and in particular GPCRs and (4) the mechanism of RNA degradation in E.coli.
 
Publications:
Engineering Human Arginase I for L-Arginine Depletion Therapy in Auxotrophic Cancers (2009) Proc Natl Acad Sci U S A
Construction and flow cytometric screening of targeted enzyme libraries (2009) Nature Protocol 4, 893-901.
Crystal Structure of the Engineered Neutralizing Antibody M18 Complexed to the Anthrax Protective Antigen (2009) J. Mol. Bio. 387, 680-93.
Disulfide bond formation by exported glutaredoxin indicates glutathione's presence in the E. coli periplasm (2009) Proc Natl Acad Sci U S A 106, 1571-7.
Engineering Antibody Fragments to Fold in the Absence of Disulfide Bonds (2009) Prot Sci. 18, 259-67.
Genetic Analysis of G Protein-Coupled Receptor Expression in Escherichia coli: Inhibitory Role of DnaJ on the Membrane Integration of the Human Central Cannabinoid Receptor (2009) Biotechnol Bioeng. 102, 357-67.
E-clonal antibodies: selection of Full-Length IgG Antibodies Using Bacterial Periplasmic Display (2008) Nature Protocol 3, 1766-77.
De novo Engineering and Evolution of Disulfide Isomerases Analogous to the Bacterial DsbC (2008) J. Biol. Chem 283, 31469-76.
Engineering a Protease that Cleaves after Sulfotyrosine (2008) Angew. Chem Intl Ed 47, 7861-3.
Engineering G Protein-Coupled Receptor Expression in Bacteria (2008) Proc Natl Acad Sci 105, 14747-8.
Efficient Production of Membrane-Integrated and Detergent-Soluble G Protein-Coupled Receptors in Escherichia coli (2008) Prot. Sci. 17, 1857-63.
Functional Plasticity of a Perioxidase Allows Evolution of Diverse Disulfide-Reducing Pathways (2008) Proc. Natl Acad. Sci. U.S.A 10, 6735-40.
Li, H., Hwang, B-Y., Lamikanthan, G., Blaber, S.I., Blaber, M., Golubkov, P. A., Ren, P., Iverson, B.L. and G. Georgiou (2008) Prot Sci 17, 664-72.
Synthetic Antibody Libraries Focused Towards Peptide Ligands (2008) J. Mol. Bio. 378, 622-33.
Highly active and selective endopeptidases with programmed substrate specificities (2008) Nature Chem. Biol. 4, 290-4.
Laboratory Evolution of E.coli Thioredoxin for Enhanced Catalysis of Protein Oxidation in the Periplasm Reveals a Phylogenetically Conserved Substrate Specificity Determinant (2008) J. Biol. Chem. 283, 840-8.
Escherichia coli tatC Mutations that Suppress Defective Twin-Arginine Transporter Signal Peptides (2007) J. Mol Bio. 374, 283-91.
Beyond Toothpicks: New techniques for Genetic Analysis in Bacteria (2007) Nature Rev Microbiol. 5, 680-8.
APEx 2-hybrid. A Quantitative Protein:Protein Interaction Assay for Antibody Discovery and Engineering (2007) Proc. Natl. Acad.. Sci. U.S.A 104, 8247-52.
Binding and Enrichment of Escherichia coli Spheroplasts Expressing Inner Membrane Tethered scFv Antibodies on Surface Immobilized Antigens (2007) Biotechnol Bioeng. 98(1), 39-47 .
A Bacterial Two-Hybrid System Based on the Twin Arginine Transporter Pathway of E.coli (2007) Protein Sci. 16, 1001-8.
A scFv Antibody Mutant Isolated in a Genetic Screen for Improved Export via the Twin Arginine Transporter Pathway Exhibits Faster Folding (2007) J. Mol. Bio 369, 631-9.
E-clonal Antibodies: Isolation of Engineered Full-Length IgG from Libraries Expressed in E. coli (2007) Nature Biotechnology 25, 563-5.
The Substrate Specificity of Kallikrein 1 and 6 Determined by Phage Display () Prot Sci 17, 664-72.
Synthetic Antibody Libraries Focused Towards Peptide Ligands () J. Mol. Bio. 378, 622-33.

 
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