The Protein Microanalysis Facility
The Facility provides peptide synthesis services utilizing a PTI Symphony synthesizer. N-terminal protein/peptide sequencing (ABI Procise 492 cLC) is perfomed in-house and internal sequencing / peptide mapping is available in conjunction with the Pharmacy Analytical Instrumentation Facility. In-gel-digestion of proteins from gel or PVDF pieces can be performed in the Facility for submission to the AIF or other facility for protein ID via MALDI-TOF-TOF or LC-MS. The Facility possesses several LC and HPLC systems that are available to trained users for both analytical and preparative purposes. (Beckman System Gold; GE Biosciences AKTA, two Bio-rad Duoflow systems). A MALDI-TOF mass spectrometer (Perseptive Biosystems Voyager) is also available for use after training. The Facility also offers electrophoresis and electroblotting services. A Mithras LB 940 multi-label reader and Phenix protein crystallization robot are also housed in the Facility.
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