University of Texas

ICMB

 

Services and Fees

Amino Acid Analysis         

$45 / run

Quantification of free amino acids  and composition for peptides and proteins

Hydrolysis

$8 automated

$10 manual

Peptides and proteins

Capillary electrophoresis           

Starting at $45 for Each analysis

CE, CZE, ACE, IEF, SDS-CE analysis of  Drugs, DNA, RNA,  proteins & peptides

Mass Spectrometric Analysis (MS)

Starting at $45 for Each analysis

Mass analysis of biomolecules using matrix assisted laser desorption MS (MALDI)

HPLC; Proteins, Peptides & oligos

$45 each analytical run $80 preparative

Analysis and separations of biomolecules using liquid chromatography

Protein/Peptide
Microsequencing

$180 (1st 10 cycles) additional $12 per cycle, $100 for 1st 5 cycles*, $100 set up.

Edman based automated sequencing of protein and peptides, N-terminal and internal.  *Only applicable if start of sequencer is scheduled before 11.00 am.

Protein Identification

$200 to 400 depending on state of sample and enzymes required

Generating of peptide ladders for proteomic research followed by isolation and sequencing of  peptides. Includes peptide mapping, digests in gels, on membranes, or in solution.

Peptide Synthesis
$17.00 at the 20 mmole scale, desalted only.  $20 to $50 per residue at the 0.25 mmole scale,  $150 purification

Synthesis of short and long peptides, cyclic peptide, peptide nucleic acids (PNA’s), peptide  mimics, and peptide conjugates

C-terminal Sequence Analysis

Call for info

Several approaches are available, e.g.
Carboxypeptidase Y digest – MS, etc.

2D electrophoresis
(to be added soon)

$100

Separation of complex protein mixtures

Native gel
Electrophoresis

$25– 50

50 to 1000 kDa

-S-S- bridge analysis

$100 - $200

Folding in proteins and peptides

Phosphopeptide analysis

$60

Analysis of phosphorylated peptides

Cross-linking

Call for info

Cross-linking of biomolecules: protein-protein, protein-DNA

BIAcore

$15 / hour,
$25 for setup

Real-time measurement of biomolecular interactions via surface plasmon resonance (SPR)

Flow Cytometer

$18 / hour

Qualitative analysis of a cell that passes through a focused laser beam

Luminometer
No fee
Chemiluminescence measurements in solution