University of Texas

ICMB


The Protein Microanalysis Facility

The Facility provides peptide synthesis services utilizing a PTI Symphony synthesizer.   N-terminal  protein/peptide sequencing (ABI Procise 492 cLC) is perfomed in-house and internal sequencing / peptide mapping is available in conjunction with the Pharmacy Analytical Instrumentation Facility. In-gel-digestion of proteins from gel or PVDF pieces can be performed in the Facility for submission to the AIF or other facility for protein ID via MALDI-TOF-TOF or LC-MS. The Facility possesses several LC and HPLC systems that are available to trained users for both analytical and preparative purposes. (Beckman System Gold; GE Biosciences AKTA, two Bio-rad Duoflow systems).  A MALDI-TOF mass spectrometer (Perseptive Biosystems Voyager) is also available for use after training. The Facility also offers electrophoresis and electroblotting services.  A Mithras LB 940 multi-label reader and Phenix  protein crystallization robot are also housed in the Facility.

For information Contact:

Michelle Gadush
gadush@mail.utexas.edu
512-471-3741
MBB 1.420
Fax 512-232-3432