University of TexasDSF

ICMB

BioanalyzerTyphoon Trio from GE Healthcare :Unites proven storage phosphor autoradiography technology with four-color, nonradioactive fluorescent labeling techniques. For DNA, RNA, and protein samples, choose from:

When one of the scanning modes is selected, the appropriate optical components are automatically activated. Typhoon™ scans gels, blots, and mounted or unmounted storage phosphor screens up to 35 × 43 cm. The instrument exhibits outstanding linearity, quantitative accuracy, and extremely low limits of detection.

Key features:

Single- and multicolor fluorescence via direct laser excitation
Fluorescence imaging (high sensitivity and high resolution)
Radio isotopic imaging – quantitate 32P, 33P, 35S, 14C and 3H
Excitation:  20mW 532 nm, 14 mW 488 nm, 10 mW 635
Emission filters: 390 nm BP, 526 nm SP, 530 nm BP, 555 nm LP, 640 nm BP

TECHNICAL SPECIFICATIONS
Scan area  35 × 43 cm 
Sensitivity (limit of detection)  Storage Phosphor: 14C (1 h exposure, 200 and 100 µm only): < 2 dpm/mm2; 32P: 5-10-fold lower than 14C
488 nm excited fluorescence:  100 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
532 nm excited fluorescence:  200 amol HEX, TMR, ROX, and 400 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
633 nm excited fluorescence:  200 amol Cy5 end-labeled DNA primer in 0.4 mm thick, 12% polyacrylamide gel with TBE buffer. 
Pixel size  1000, 500, 200, 100, 50, 25, and 10 µm, selectable. 
Spatial resolution  Autoradiography: 2 line pairs/mm for lines drawn with 14C ink.
Blue-excited fluorescence: 10 line pairs/mm
Green-excited fluorescence: 10 line pairs/mm
Red-excited fluorescence: 10 line pairs/mm 
Uniformity  ± 5% over entire scan area. 
Pixel accuracy  ± 0.15% 
Data format  16-bit (65 536 levels), TIFF 
Linear dynamic range and linearity  Five orders of magnitude (100 000:1) with less than 7.5% relative standard deviation for entire dynamic range. 
Red light source  Type: 10 mW Helium-Neon laser Estimated average lifetime: ~ 10 000 h Wavelength: 632.8 nm 
Green light source  Type: 20 mW solid-state doubled frequency SYAG laser  Estimated average lifetime: ~ 10 000 h Wavelength: 532 nm 
Blue light source  Type: 30 mW all blue lines Argon ion laser  Estimated average lifetime: ~ 5000 h Wavelength: 488 nm (20 mW) and 457 nm (4 mW)