Typhoon Trio from GE Healthcare :Unites proven storage phosphor autoradiography technology with four-color, nonradioactive fluorescent labeling techniques. For DNA, RNA, and protein samples, choose from:
- Storage phosphor autoradiography
- Direct blue-excited fluorescence (457, 488 nm)
- Direct green-excited fluorescence (532 nm)
- Direct red-excited fluorescence (633 nm)
- Chemiluminescence
Key features:
Single- and multicolor fluorescence via direct laser excitation
Fluorescence imaging (high sensitivity and high resolution)
Radio isotopic imaging – quantitate 32P, 33P, 35S, 14C and 3H
Excitation: 20mW 532 nm, 14 mW 488 nm, 10 mW 635
Emission filters: 390 nm BP, 526 nm SP, 530 nm BP, 555 nm LP, 640 nm BP
- Versatile system handles gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, and even microarrays.
- Powerful excitation sources and innovative high-quality confocal optics allow for the sensitive detection of low-abundance targets.
- Red-, green-, and blue-excitation wavelengths and a wide choice of emission filters enable imaging of an extensive variety of fluorophores.
- Automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample ensuring accuracy of analysis, increasing throughput, and saving time.
- Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 125I, 32P, 33P, 35S, and other sources of ionizing radiation.
- Highly sensitive optics enable direct chemiluminescent imaging without intermediate exposure to films or screens.
TECHNICAL SPECIFICATIONS Scan area 35 × 43 cm Sensitivity (limit of detection) Storage Phosphor: 14C (1 h exposure, 200 and 100 µm only): < 2 dpm/mm2; 32P: 5-10-fold lower than 14C
488 nm excited fluorescence: 100 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
532 nm excited fluorescence: 200 amol HEX, TMR, ROX, and 400 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
633 nm excited fluorescence: 200 amol Cy5 end-labeled DNA primer in 0.4 mm thick, 12% polyacrylamide gel with TBE buffer.Pixel size 1000, 500, 200, 100, 50, 25, and 10 µm, selectable. Spatial resolution Autoradiography: 2 line pairs/mm for lines drawn with 14C ink.
Blue-excited fluorescence: 10 line pairs/mm
Green-excited fluorescence: 10 line pairs/mm
Red-excited fluorescence: 10 line pairs/mmUniformity ± 5% over entire scan area. Pixel accuracy ± 0.15% Data format 16-bit (65 536 levels), TIFF Linear dynamic range and linearity Five orders of magnitude (100 000:1) with less than 7.5% relative standard deviation for entire dynamic range. Red light source Type: 10 mW Helium-Neon laser Estimated average lifetime: ~ 10 000 h Wavelength: 632.8 nm Green light source Type: 20 mW solid-state doubled frequency SYAG laser Estimated average lifetime: ~ 10 000 h Wavelength: 532 nm Blue light source Type: 30 mW all blue lines Argon ion laser Estimated average lifetime: ~ 5000 h Wavelength: 488 nm (20 mW) and 457 nm (4 mW)