Fragment Analysis :Fluorescently labeled fragments are detected using the Applied Biosystems 3130XL or 3730 Genetic Analyzer and then interpreted using the GeneMapper or GeneMarker analysis software. Five different colored fluorescent dyes can be detected in one sample. One of the dye colors is used for a labeled size standard that is added to each lane to allow comparison of samples from lane to lane or gel to gel. The analysis software uses the size standard to create a standard curve for each lane and then determines the length of each dye-labeled fragment by comparing it with the standard curve for that specific lane. Accuracy of less than one base difference is seen between sample replicates. Higher throughput can be achieved by multiplexing different dyes and fragment sizes together.
Key features:
Dyes detected: DS-30 (6FAM, HEX, NED, ROX), DS-32 (5FAM, JOE, NED, ROX),
DS-33 (6FAM, VIC, NED, PET, LIZ)
Rox labeled size standards available
Analysis software GeneMapper and GeneMarker available
Applications:
Micro satellite Analysis
AFLP Analysis (amplified fragment length polymorphisms)
SNP Analysis (single nucleotide polymorphisms)
SNPlex (multiplex up to 48 SNP’s per lane)
Gene Expression Profiling
Mutation Detection
For fragment analysis we have available GeneMarker by SoftGenetics and GeneMapper from Appliedbiosystems. These programs run as network shared programs that run on your PC but get authorization from our server. We have 3 licenses for GenMarker and 4 licenses for GeneMapper. Please contact Cecil Harkey for help.
Fragment Analysis Handout
GeneMarker Manual
GeneMapper AFLP Guide
GeneMapper Microsatellite Guide
GeneMapper SNPlex Guide
GeneMapper Quick Reference Guide
GeneMapper Reference Guide
