Sequencing Troubleshooting

My sample failed, why?
The #1 problem with samples that fail is template concentration. If you're having trouble with the spec in your lab, come down and use our Nanodrop. It's free and only takes a minute of your time and a microliter of your sample! Having the correct template concentration initially can save you a lot of time and money later.
Contamination is another reason why samples fail. Contamination can be in the form of the following and in either your template, primer or both:
Two templates present in one sample
RNA
Proteins

To ensure that you do not have two templates in your sample, you can run your sample out on a gel.
This is an example of a dirty sequence. This can be caused by any of the above problems.

To fix this problem you should do one or all of the following:
1. Run you sample out on a gel to make sure that there is only one template present.
2. Check your primer sequence and make sure there aren’t two primer binding sites on your template.
3. Clean up your sample again to try and get rid of any contamination.

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Please visit one of the Following Pages:

http://genomeold.wustl.edu/tools/3700help/seq_tutorial/frame.htm

If you are having problems sequencing, please come and talk with Cecil or Allyson. We really care about your samples and will make every effort to help you achieve sequencing success.