Faculty

M. Stephen  Trent

Trent , M. Stephen
Professor in Molecular Biosciences

E-mail

Main Office: NMS 3.118
Phone: (512) 232-8371

Alternate Office: NMS 3.236
Phone: (512) 232-8372

Mailing Address:
The University of Texas at Austin
Department of Molecular Biosciences
2506 Speedway Stop A5000
Austin, TX 78712-1191


Research Lab Students:
    Graduate Student
  • Cullen, Thomas - Graduate Student
  • Needham, Brittany - Graduate Student
  • Nowicki, Emily - Graduate Student
  • Pride, Aaron - Graduate Student
    Post Doctoral
  • Giles, David - Post Doctoral
  • Hankins, Jessica - Post Doctoral
  • Herrera, Carmen M - Post Doctoral
    Staff
  • Ivanov, Petko - Staff


  • Research Summary:

    In order to survive, bacteria must quickly respond to changes in the surrounding environment. We are interested in how diverse environmental stimuli promote changes in conserved microbial structures found on the surface of pathogenic bacteria. Currently, our laboratory focuses on the remodeling of lipopolysaccharides or LPS. LPS, also referred to as endotoxin, is the major surface component of Gram-negative bacteria and represents one of the microbial molecular signals responsible for activation of the host innate immune system. Our goal is to identify and characterize the molecular mechanisms necessary for the remodeling of LPS, and to determine how alteration of LPS contributes to evasion of the innate immune response during infection. Several pathogenic bacteria are under investigation in our laboratory including Vibrio cholerae, Campylobacter jejuni, Helicobacter pylori, Salmonella typhimurium, and pathogenic strains of Escherichia coli. Understanding how bacteria remodel their cell surface is of fundamental importance and may yield new therapeutic strategies for intervention in bacterial infections.

    Research Images:


     
    Publications:
    2014Whitfield C, Trent MS, Biosynthesis and Export of Lipopolysaccharides, Annual Rev. of Biochem.in press.
    2014Rubin EJ, O'Brien JP, Ivanov PL, Brodbelt , Identification of a broad family of lipid A acyltransferases with non-canonical substrate specificity, Mol. Micro.in press.
    2013Carpenter CD, Cooley BJ, Needham BD, Fisher CR, Trent MS, Payne SM, The Vps/VacJ (Mla) ABC transporter is required for intercellular spread of Shigella flexneri, Infection and Immunityin press.
    2013Henderson JC, O'Brien JP, Brodbelt JS, Trent MS, Isolation and chemical characterization of lipid A from the outer membrane of gram-negative bacteria, J. Vis. Exp. 79:e50623.
    2013Rubin EJ, Trent MS, Colonize, evade, fluorish: How glyco-conjugates promote virulence of Helicobacter pylori, Gut Microbiesin press.
    2013Madsen JA, Xu H, Robinson MR, Horton AP, Shaw JB, Giles DK, Kaoud TS, Dalby KN, Trent MS, Brodbelt JS, High-througput database search and large-scale negative polarity liquid chromatography-tandem mass spectrometry with ultraviolet photodissociation for complex proteomic samples, Mol. & Cell. Proteomics 9:2604-14.
    2013Pride AP, Herrera CM, Guan Z, Giles DK, Trent MS, The outer surface lipoprotein VolA mediates utilization of exogenous lipids by Vibrio cholerae, MBio 14:5-13.
    2013Needham BD, Trent MS, Fortifying the barrier: the impact of lipid A remodeling on bacterial pathogenesis, Nature Micro. Rev. 11:467-81.
    2013Gattis SG, Chung HS, Trent MS, Raetz CR, The origin of 3-amino-3,8-dideoxy-D-manno-octulosonic acid (Kdo8N) in the lipopolysaccharide of Shewanella oneidensis, J. Biol. Chem. 288:9215-24.
    2013Hankins JV, Madsen JW, Needham BS, Brodbelt JS, Trent MS , The outer membrane of Gram-negative bacteria: Lipid A isolation and characterization, Methods Mol. Bio. 966:239-58.
    2013Moreira CG, Herrera CM, Needham BD, Parkera CT, Libby SJ, Fang FC, Trent MS, Sperandio V, Virulence and stress-related periplasmic protein (VisP) in bacterial/host 2 associations, PNAS 110:1470-5.
    2013Needham BD, Carroll S, Giles DK, Georgiou G, Whiteley M, Trent MS, Modulating the Innate Immune Response by Combinatorial Engineering of Endotoxin , PNAS 110:1464-9.
    2013Cullen TW, O'Brien JP, Hendrixson DR, Giles DK, Hobb RI, Thompson SA, Brodbelt JS, Trent MS, EptC of Campylobacter jejuni mediates phenotypes involved in host interactions and virulence, Infection and Immunity 81:430-40.
    2012Maue AC, Mohawk KL, Giles DK, Poly F, Ewing CP, Jiao Y, Lee G, Ma Z, Monteiro MA, Hill CL, Ferderber JS, Porter CK, Trent MS, Guerry, P, The polysaccharide capsule of Campylobacter jejuni 81-176 modulates the host immune response, Infection and Immunity 81:665-72.
    2012Gellatly SL, Needham B, Madera L, Trent MS, Hancock RE, The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation, Infection and Immunity 80:3122-31.
    2012Hankins JV, Madsen JW, Giles DK, Brodbelt JS, Trent MS, Amino acid addition to Vibrio cholera LPS establishes a link between surface remodeling in Gram-negative and Gram-positive bacteria., PNAS 109:8722-7.
    2012Cullen TW, Madsen JA, Ivanov PL, Brodbelt JS, Trent MS, Characterization of a unique modification of the flagellar rod protein FlgG by the Campylobacter jejuni lipid A phosphoethanolamine transferase, linking bacterial locomotion and antimicrobial peptide resistance, J. Bio. Chem. 287:3326-36.
    2011Cullen TW, Giles DK, Wolf LN, Ecobichon C, Boneca IG, Trent MS , Helicobacter pylori Versus The Host: Remodeling Of The Bacterial Outer Membrane Is Required For Survival In The Gastric Mucosa , PLoS Pathogens 7:1-18.
    2011Hankins JV, Madsen JA, Giles DK, Childers BM, Klose KE, Brodbelt JS, Trent, MS, Elucidation of a novel Vibrio cholerae lipid A secondary hydroxy-acyltransferase and its role in innate immune recognition, Mol. Micro. 81:1313-29.
    2011Madsen JA, Cullen TW, Trent MS, Brodbelt JS, IR and UV Photodissociation as Analytical Tools for Charaacterizing Lipid A Structures, Anal. Chem. 83:5107-13.
    2011Arroyo LA, Herrera CM, Fernandez L, Hankins JV, Trent MS, Hancock RE, The pmrCAB operon mediates polymyxin resistance in Acinetobacter baumannii ATCC 17878 and clinical isolates through phosphoethanolamine modification of lipid A, Antimicrob Agents Chemother 55:3743-51.
    2011Giles DK, Hankins JV, Guan Z, Trent MS, Remodeling of Vibrio cholerae membranes by incorporation of exogenous fatty acids from host and acquatic environments, Mol. Micro. 79:716-28.
    2010Stead CM, Zhao J, Raetz CRH, Trent MS, Removal of the outer Kdo from Heliobacter pylori lipopolysaccharide and its impact on the bacterial surface , Mol. Micro. 78:837-52.
    2010Herrera CM, Hankins JV, Trent MS, Activation of PmrA inhibits LpxT-dependent phosphorylation of lipid A promoting resistance to antimicrobial peptides, Mol. Micro. 76:1444-60.
    2010Cullen TW, Trent MS, A link between the assembly of flagella and lipooligosaccharide of the Gram-negative bacterium Campylobacter jejuni, PNAS 107:5160-5.
    2009Rutten, L., Mannie, J.-P. B. A., Stead, C. M., Raetz, C. R. H., Reynolds, C. M., Bonvin, A. M. J. J., Tommassen, J., Egmond, M. R., Trent, M. S.*, and Grois, P*. *Joint Corresponding Authors, Active site architecture and catalytic mechanism of the lipid A deacylase LpxR of Salmonella typhimurium, PNAS 106:1960-4.
    2009Hankins, J. V. and Trent, M. S., Phosphorylation of Kdo is required for secondary acylation of the V. cholerae lipopolysaccharide, J. Bio. Chem. 284:25804-12.
    2008Kline, T., Trent, M. S., Stead, C. M., Lee, M. S., Sousa, M. C., Felise, H. B., Nguyen, H. V., and Miller, S. I., Synthesis of and Evaluation of Lipid A Modification by 4-Substituted 4-Deoxy Arabinose Anaolgs as Potential Inhibitors of Bacterial Polymyxin Resistance, Biorg. & Med. Chem. Letters 18:1507-10.
    2008Touze, T., Tran, A. X., Hankins, J. V., Mengin-Lecreulx, D. and Trent, M. S., Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate, Mol. Micro. 67:264-277.
    2008Tran, A. X., Trent, M. S., and Whitfield C., The LptA protein of Escherichia coli is a periplasmic lipid A binding protein involved in the lipopolysaccharide export pathway, J. Biol. Chem. 283:20342-9.
    2008Stead, C. M., Beasley, A., Cotter, R. J., and Trent, M. S., Deciphering the unusual acylation pattern of Helicobacter pylori lipid A, J. Bacteriology 190:7012-21.
    2007Raetz, C. R. H., Reynolds, C. M. Trent, M. S. and Bishop, R. E., Lipid A modification systems of Gram-negative bacteria, Annual Rev. of Biochem. 76:295-329.
    2006Ernst, R. K., Adams, K. N., Moskowitz, S. M., Kawasaki, K., Stead, C. M., Trent, M. S. and Miller, S. I., The Pseudomonas aeruginosa Deacylase (PagL) required for synthesis of cystic fibrosis-specific lipid A, J. Bacteriology 188:191-201.
    2006Trent, M. S., Stead, C. M., Tran, A. X., and Hankins, J. V. , Diversity of Endotoxin and its Impact on Pathogenesis, J. of Endotoxin Res. 12:205-223.
    2006Tran, A. X., Whittimore, J. D., Wyrick, P. B., McGrath, S. C., Cotter, R. J. and Trent, M. S., The Lipid A 1-Phosphatase of Helicobacter pylori is Required for Antimicrobial Peptide Resistance, J. Bacteriology 188:4531-4541.
    2006Trent, M. S., Stead, C. M., Tran, A. X., and Hankins, J. V, Diversity of Endotoxin and its Impact on Pathogenesis, J. of Endotoxin Res. 12:205-223.
    2006Tran, A. X., Whittimore, J. D., Wyrick, P. B., McGrath, S. C., Cotter, R. J. and Trent, M. S. , The Lipid A 1-Phosphatase of Helicobacter pylori is Required for Antimicrobial Peptide Resistance, J. Bacteriology 188:4531-4541.
    2006Reynolds, C. M., Ribeiro, A. A., McGrath, S. C., Cotter, R. J., Raetz, C. R. H., and Trent, M. S. , An outer membrane enzyme in Salmonella typhimurium that removes the 3ยด-acyloxyacyl moiety of lipid A, J. Biol. Chem. 281:21974-87.
    2005Tran, A. X., Stead, C. M., and Trent, M. S., Remodeling of Helicobacter pylori lipopolysaccharides, J. of Endotoxin Res. 11:161-6.
    2005Tran, A. X., Lester, M. E., Stead, C. M., Raetz, C. R. H., Maskell, D. J., McGrath, S. C., Cotter, R. J., and Trent, M. S., Resistance to the antimicrobial peptide polymyxin requires secondary myristoylation of Gram-negative bacterial lipid A, J. Biol. Chem. 280:28186-94.
    2005Stead, C. M., Tran, A. X., Ferguson D. A. Jr., McGrath, S. C., Cotter, R. J. and Trent, M. S., A Novel Kdo-Hydrolase that removes the outer Kdo sugar from Helicobacter pylori lipopolysaccharide, J. Bacteriology 187:3374-83.
    2004Trent, M. S, Biosynthesis, Transport, and Modification of Lipid A, Biochemistry and Cell Biology 82:71-86.
    2004Tran, A. X., Karbarz, M. J., Wang, X., Raetz, C. R., McGrath, S. C., Cotter, R. J. and Trent M. S. , Periplasmic Cleavage and Modification of the 1-Phosphate Group of Helicobacter pylori Lipid A, J. Biol. Chem. 279:55780-91.

     
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